Three people died in a 21-vehicle vehicle accident on I-75 in Columbia County early Sunday morning.

According to a Florida Highway Patrol (FHP) release, the multi-vehicle collision occurred around 12:40 a.m. at the northbound Lake City/High Springs 414 exit ramp. The roadway was closed for several hours to clear the wreckage and an FHP investigation is ongoing.

The FHP released a limited report Sunday evening and more details are forthcoming.

“One of the vehicles involved was a Greyhound bus, and I understand it was loaded with passengers,” FHP spokesman Pat Riordan said in a phone interview Monday morning. “Since there were three people killed, we are doing a homicide investigation. I would imagine it’s going to be very lengthy due to the number of vehicles, the number of persons involved, and the enormous amount of information our investigators have to gather.”

Editor’s note: This is a developing story. It has been updated and may be again as more information is released.